Mark T. Nelson, PhD
Chair and Distinguished Professor
My laboratory explores the control of endothelial and smooth muscle cell function by the cell membrane. A combined approach utilizing; single cell isolation, single channel recordings, intracellular calcium measurements, laser scanning confocal microscopy, diameter and membrane potential measurements in intact pressurized arteries, and protein expression – is used to examine the physiological properties of calcium and potassium channels. Activity at calcium and potassium channels allows active neurons in the brain to call for increased blood flow; sympathetic nerves to signal changes to blood pressure; and signaling in the control of urinary bladder function. Drug treatments for cardiovascular disorders, such as hypertension, angina and stroke target these areas. Of particular interest is cerebrovascular research of small vessel diseases (SVDs) in the brain. SVDs account for 30% of ischemic strokes and about 40% of cognitive decline and disability or dementia. Currently there are no specific treatments for SVDs or preventative therapies. By studying the mechanisms involved in both health and disease, we hope to develop therapeutic targets for both treatment and prevention.
Thomas Heppner, PhD
My research identifies fundamental processes that underlie the regulation of smooth muscle from urinary bladder and vascular tissues. This involves the study of ion channels and calcium signals from smooth muscle that affects membrane potential. I use a variety of techniques, including force measurements, calcium imaging, nerve recordings and electrophysiology.
David Hill-Eubanks, PhD
My primary research contribution is the molecular perspective and iconoclastic mindset I bring to the various projects of the Nelson laboratory. When I’m not poking the dominant paradigm with a stick, I contribute writing and editorial expertise to a range of laboratory products. In this capacity, I have acted as lead editor and co-writer of numerous successful NIH grants, international collaborative research grants, and post-doctoral fellowships. I also routinely edit papers submitted by the Nelson laboratory and assist Mark in reviewing papers and grant proposals.
Masayo Koide, PhD
Using experimental techniques from molecular level to whole animal (e.g. in vivo measurements of CBF and vivo astrocyte Ca2+ imaging), my goal is to understand the mechanisms of cerebral blood flow (CBF) regulation, specifically in the context of dysregulation of neurovascular communication in pathological conditions such as hypertension and hemorrhagic stroke.
Amreen Mughal, PhD
My overall research goal is to evaluate mechanisms involved in regulation of blood flow in the brain and how vascular endothelial and contractile (smooth muscle and pericytes) cells contribute in neurovascular coupling. I am also interested to explore mechanisms associated with impaired cerebral blood flow in Alzheimer’s disease. With the use of in vivo imaging approaches in awake and anesthetized mouse models with supportive ex vivo techniques and novel analysis approaches, my goal is to provide better understanding about neurovascular coupling mechanisms in health and Alzheimer’s disease.
Grant Hennig, PhD
My main interest involves developing ways to better describe and understand how intra/inter-cellular signaling molecules spread through biological networks to alter the behavior of various organs, such as blood vessels and the bladder. Multi-dimensional image analysis requires novel spatio-temporal approaches which I design and refine using the custom-written Volumentry platform.
Visiting Assistant Professor
Maria Sancho-Gonzalez, PhD
My primary goal is to better understand how blood flows within the dense vascular network supplying the brain. I am particularly interested in defining the still incompletely understood biophysical properties and ion channel signatures of the integral components of brain capillaries—endothelial cells and pericytes—which contribute to vascular function and cerebral blood flow control. My current research focuses on exploring the electro-metabolic sensing properties of capillary cells and their potential impact on cerebral hemodynamics in health and under conditions in which there is a mismatch between tissue blood/oxygen supply and demand.
Gerry Herrera, PhD
My primary interest is understanding factors that control smooth muscle excitability and contractility. I apply techniques from the single cell level up to the whole animal. Areas of focus include urogenital, gastrointestinal, and cardiovascular systems. I am also very interested in laboratory instrumentation development, and I direct R&D for my family businesses Med Associates, Catamount Research and Development, and Living Systems Instrumentation. As such, I am involved with developing instrumentation for behavioral neurosciences and many areas of physiology.
Nicholas Klug, PhD
My overall research interest is to understand how endothelial and contractile cells in the smallest blood vessels of the brain and retina regulate blood flow. I am also interested in urinary bladder function, particularly the adenosine signaling pathways which regulate normal and disordered bladder function. I utilize single cell to whole animal techniques to understand cellular and molecular mechanisms which lead to normal and diseased blood vessel and urinary bladder function.
Maria Noterman, PhD
My past PhD research investigated the cell-type specific roles of Cav1.2a1 in the brain using molecular and cellular biological techniques, specializing in metabolomics and mitochondrial physiology. I aspire to apply my background in cellular energetics to neurovascular coupling in the Nelson laboratory.
Saúl Huerta de la Cruz, PhD
My overall interest is to understand the electrophysiological features and the functional interaction among the cells that comprise the neurovascular unit. From a single-cell approach involving patch-clamp electrophysiology to whole animal in vivo experimentation (two-photon imaging and cerebral blood flow measurements) my primary goal is to provide fundamental insights into the complex mechanisms underlying the neurovascular coupling (NVC). As a pharmacologist, I am also interested in identifying the molecular mechanisms driving neurovascular dysfunction in disease conditions.
Neuroscience PhD Student
I am broadly interested in the neurovascular underpinnings of learning and memory, as well as how dysfunction of the neurovascular network contributes to the pathogenesis of neurodegenerative diseases and age-related cognitive decline.
Nelson Lab Senior Technician
Daniel Enders, MS
Nelson/Herrera Lab Senior Technician
As Lab Technicians, we are in charge of day-to-day management of the lab including orders of chemicals & equipment, and overseeing laboratory safety procedures. We maintain 25+ transgenic mouse lines, and are responsible for over 800 individual mice and one very important coffee maker. We assist researchers by developing mice varieties with genetic combinations they need with strict adherence to IACUC protocols.
Klug/Nelson Projects - Supported by Totman Medical Trust Funding
I am working with Dr. Klug to investigate the role of adenosine signaling in blood flow regulation in the brain and retina. We are also interested in the role of pericytes in normal and diseased blood flow in the brain. The techniques I am using to address these questions include brain vessel pressure myography and in vivo imaging of the brain vasculature using 2-photon microscopy.
Herrera/Heppner Projects - Supported by Herrera/Heppner Funding
We are studying how the specialized urothelial cells that line the lumen of the urinary bladder modulate bladder function. Our approach is to use isolated strips of urinary bladder to examine how strips with the urothelium intact respond to various experimental perturbations as compared to bladder strips in which the urothelium has been physically removed. In this way, we can better understand how the urothelium contributes to regulation of urinary bladder contractility. Our findings will help us determine if the urothelium could serve as a therapeutic target in developing treatments of bladder dysfunctions such as overactive bladder and urinary incontinence.
Herrera/Heppner Projects- Supported by Herrera/Heppner Funding
I am working with Dr. Herrera to investigate how the urinary bladder responds to changes in pressure and volume to develop a better understanding of how sensory information from the bladder is relayed to the central nervous system to indicate when the bladder is full. We are using an in vitro model consisting of the lower urinary tract, and we control for intraluminal pressure and volume.
We also are investigating the contributions of various genetically encoded cell types within the lower urinary tract to generate a better understanding of each cell type and the communication amongst the various cells that make up the wall of the bladder. We do this by using mouse models that are genetically encoded to express calcium indicators. By understanding the mechanisms that generate sensory information in the lower urinary tract, we will be better equipped to design and develop therapeutic strategies for treating voiding disorders.
Koide Projects - Supported by Koide Funding
I work with Dr. Masayo Koide on the project examining the effects of elevated plasma aldosterone (termed hyperaldosteroemia) on cerebral blood flow and cognitive function in mice, as a possible risk factor for dementia. I am excited to apply the past few years of classroom lectures to real-world lab experiences utilizing cutting-edge scientific practices.
Nelson/Noterman-Soulinthavong Projects- Supported by Totman
Medical Trust Funding
I am working with Dr. Maria Noterman-Soulinthavong to understand the role that ATP contributes to vessel function in the brain, and how that is implicated in small vessel diseases. Brain small vessel diseases are a major health problem, as they precede many cases of stroke and dementia and lack disease-altering treatment. Our lab recently found that deficits in a mouse model of small vessel disease were rescued by providing ATP to endothelial cells from capillaries. We hypothesize that the mechanism of some small vessel diseases is due to impaired endothelial cell energy production. To test this hypothesis, I am using techniques such as brain vessel pressure myography, and isolating capillary endothelial cells from the brain to be used in patch-clamp electrophysiology to test how energy availability affects these vascular functions.
The University of Vermont
Larner College of Medicine
Department of Pharmacology
89 Beaumont Avenue
Given Building B333
Burlington, Vermont 05405